Acclimatization of Micro Shoot-tip Cutting Red Palestine Fig on Several Planting Media Compositions

Abstract

Acclimatization is a critical stage in plant micropropagation. Many plantlets resulting from tissue culture cannot
survive when acclimatized. External environmental conditions will affect the ability of acclimatized plants to survive.
Apart from climatic conditions (temperature and sun intensity), the type of media and its composition of the planting media
can also influence the ability of plantlets to survive and grow. This research aims to: 1) see effect of media planting
composition on acclimatization of fig micro shoot-tip cutting, and 2) find the best composition of acclimatization media
treament for the success and growth of acclimatized fig from micro shoot-tip cutting. The research was carried out using a
Randomized Block Design with 7 treatments, and each treatment was repeated 4 times. Each experimental unit was planted
with 6 cuttings of fig plants resulting from in vitro multiplication. The data were subjected to analysis of variance, and
differences between acts were tested with Tukey Test at the 5% level. The results showed: 1) The media planting
composition were success to influence acclimatization of fig micro shoot-tip cutting, 2) The best composition of
acclimatization media treament for the success and growth of fig micro shoot-tip cutting is top soil+cocopeat, and 3) The
highest percentage of survival rate on fig micro shoot-tip cutting acclimatization (75%) obtained on top soil+cocopeat
media followed by top soil media (45%).