Bio-Invigoration of DETAP-1 Soybean (Glycine max) Seeds Through PGPR Application

Abstract

Soybeans are a source of plant-based protein and a staple food ingredient, especially in Indonesia. When
stored for extended periods, soybean seeds may experience quality deterioration, which can impact their productivity. An
effort to improve the quality of deteriorated soybean seeds is through bio-invigoration treatment. The goal of bioinvigoration

is to enhance seed metabolism through imbibition. One method that can be used as bio-invigoration
treatment is the application of plant growth-promoting rhizobium (PGPR). This research was conducted from May to
July 2024 at the Seed Analysis Laboratory, Politeknik Negeri Lampung. The research was arranged in a Completely
Randomized Design (CRD) with two factors, namely concentration (K) and soaking duration (L). For the concentration
factor, there were four levels: 0% (K0), 6% (K1), 12% (K2), and 24% (K3), and the soaking duration factor consisted of
two levels: 3 hours (L1) and 6 hours (L2). The factors were combined and repeated four (4) times, resulting in 32
experimental units. Data were analyzed using variance analysis at 5% and 1% significance levels, and if significant
differences were observed, further testing was conducted using the Least Significant Difference (LSD) test at the 5%
level. The results showed that PGPR can improve the viability and vigor of Detap-1 soybean seeds that have experienced
deterioration. Treatment with 12% PGPR with a 6-hour soaking duration can increase the seed's germination by
approximately 11.33%, from the initial germination rate of 68% to 80%.